Separation and Purification Techniques in Biotechnology

This book describes separation and purification techniquesùadsorption, ion exchange and liquid chromatography on solid supportsùused for fermentation and biochemical feedstreams. Emphasis is placed on basic sorption theory, laboratory evaluation techniques, sorptive materials and their characteristics, scale-up of laboratory techniques, and their industrial applications. Each chapter contains specific examples illustrating the use of purification techniques in biotechnology processes.

Introduction 1.1 Fermentation Broth 1.2 Recovery Unit Operations 1.3 Recovery Processes 1.4 References Adsorption 2.1 Introduction 2.2 Adsorption Theory 2.3 Types of Adsorbents 2.4 Laboratory Evaluation of Adsorbents 2.5 Adsorbent Operations 2.6 Process Considerations 2.7 Adsorption Applications 2.8 References Ion Exchange 3.1 Introduction 3.2 Theory 3.3 Ion Exchange Materials and Their Properties 3.4 Laboratory Evaluation Resin 3.5 Ion Exchange Processes 3.6 Ion Exchange Operations 3.7 Process Considerations 3.8 Biotechnology Applications Appendix 3.9 References Column Chromatography Processes 4.1 Introduction 4.2 Classifications of Chromatography 4.3 Chromatographic Materials 4.4 Theory 4.5 Laboratory Techniques 4.6 Scale-Up Procedures 4.7 Industrial Systems 4.8 Chromatography Applications References Affinity Chromatography 5.1 Introduction 5.2 Theory of Affinity Chromatography 5.3 Affinity Chromatography Materials 5.4 Laboratory Practices 5.5 Scale-Up of Laboratory Procedures 5.6 Affinity Chromatography Operations 5.7 Affinity Chromatography Applications 5.8 References Index