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Cell Polarity and Morphogenesis, Volume 139


Author: Thomas Lecuit

Publisher: Academic Press

Publish Date: 20th February 2017

ISBN-13: 9780128094570

Pages: 236

Language: English



Cell Polarity and Morphogenesis, the latest volume in the Methods in Cell Biology series, looks at cell polarity and morphogenesis. Edited by leaders in the field, this volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.

Table of Contents

Series Editors Chapter 1. A user’s guide for characterizing plasma membrane subdomains in living cells by spot variation fluorescence correlation spectroscopy Introduction 1. Building a Sensitive and Robust Homemade Spot Variation Fluorescence Correlation Spectroscopy/Fluorescence Recovery After Photobleaching Setup 2. Sample Preparations and Labeling 3. Fluorescence Correlation Spectroscopy Recording 4. Data Analysis and Interpretation Conclusion Chapter 2. Investigating symmetry breaking in yeast: From seeing to understanding Introduction 1. Genetic Tools 2. Microscopy-Based Methods to Visualize Cell Polarization Conclusion and Future Perspective Notes Chapter 3. Precise tracking of the dynamics of multiple proteins in endocytic events Introduction 1. Rationale 2. Sample Preparation 3. Fluorescence Microscopy 4. Image Processing 5. Tracking of the Endocytic Spots 6. Correction of Dual Channel Trajectory Pair Alignment 7. Generation of Average Trajectories and Alignment of the Average Trajectories Conclusion Chapter 4. Quantitative approaches for the study of microtubule aster motion in large eggs Introduction 1. Three-Dimensional Tracking of Microtubule Asters in Sea Urchin Embryos 2. Laser Ablation of Astral Microtubules and In Situ Immunostaining Conclusions Chapter 5. Dissection and characterization of microtubule bundles in the mitotic spindle using femtosecond laser ablation Introduction 1. Sample Preparation 2. Imaging 3. Laser Ablation 4. Image Analysis Conclusion Chapter 6. Fluorescence eXclusion Measurement of volume in live cells Introduction 1. Method 2. FXm Pitfalls and Limitations 3. Discussion Chapter 7. Quantitative methods to study epithelial morphogenesis and polarity Introduction 1. Biological Toolbox 2. Imaging Toolbox 3. Image Processing Toolbox 4. Measurements Concluding Remarks Chapter 8. Probing tissue interaction with laser-based cauterization in the early developing Drosophila embryo Introduction 1. Materials 2. Methods Conclusion Chapter 9. Optogenetic inhibition of apical constriction during Drosophila embryonic development Introduction 1. Methods Conclusion Chapter 10. Micropipette aspiration: A unique tool for exploring cell and tissue mechanics in vivo Introduction 1. Building and Preparing a Microaspiration Setup 2. Subcellular Surface Tension Mapping 3. Mechanical Properties of Tissues General Conclusions Supplementary Data Chapter 11. In-vivo analysis of morphogenesis in plants Introduction 1. Measuring the Growth: Live Imaging of Growing Tissues at Cellular Resolution 2. Probing the Wall Stiffness and Anisotropy Conclusions and Perspectives: Beyond Quantitative Live Imaging Acknowledgments